Composite

Part:BBa_K3276005

Designed by: Jackie Shane   Group: iGEM19_CSL_Pittsburgh   (2019-10-16)


Cytochrome P450 driven by a constitutive promoter

The Cytochrome P450 gene CYP6AY1 from the brown planthopper was inserted following Part:BBa_S05355 with constitutive J23119 promoter and B0034 RBS. This gene allows the brown planthopper to be resistant to the pesticide imidacloprid. Part:BBa_S05355 contains the constitutive promoter J23119 with RBS B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1180
    Illegal SapI.rc site found at 386


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